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KMID : 0861020120270060001
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Korea Journal of Herbology
2012 Volume.27 No. 6 p.1 ~ p.6
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The effect of Saururus chinensis Baill against oxidative damage and inflammation
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Hwang Dong-Ryeol
Jeong Jin-Boo
Eo Hyun-Ji
Hong Se-Chul
Yoo Ji-Hyun
Lee Kun-Hee
Kim Bo-Ram
Koo Jin-Suk
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Abstract
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Objectives: ROS are involved in a wide spectrum of diseases including chronic inflammation and cancer. S.chinensis Baill, a perennial herb commonly called Chinese lizard’s tail or Sam-baek-cho in Korea, is used for the treatment of edema and inflammatory diseases in the Oriental folk medicine. In this study, we investigated the antioxidant activities and anti-inflammatory effects of the two extracts, water(WE) and ethyl acetate(EAE) from S.chinensis Baill.
Methods: Anti-oxidant activity was evaluated using Fe2+ chelating and hydroxyl radical scavenging assay. DNA cleavage assay, and western blot and immunostaining for phospho-p65 were performed to evaluate anti-oxidative effect. Anti-inflammatory effect was performed using NO generation assay and western blot in LPS-stimulated RAW264.7 cell.
Results: In Fe2+ chelating activity and hydroxyl radical scavenging activity, WE showed more strong scavenging activity for hydroxyl radical than EAE. WE scavenged hydroxyl radical by 12% at 3.2 ㎍/ml, 21% at 16 ㎍/ml, 32% at 80 ㎍/ml, 66% at 400 ㎍/ml and 82% at 2000 ㎍/ml, respectively. In addition, WE showed more strong chelating activity than EAE. WE chelated Fe2+ ion by 1.1% at 3.2 ㎍/ml, 8.2% at 16 ㎍/ml, 26.3% at 80 ㎍/ml, 72% at 400 ㎍/ml and 89% at 2000 ㎍/ml, respectively. Also, WE inhibited oxidative damage via its anti-oxidant activity. In anti-inflammatory effect, EAE inhibited NO production and iNOS expression. In addition EAE suppressed the NF-κB and MAPK signaling pathway in LPS-stimulated RAW 264.7 cells.
Conclusions: Together, these data indicate that S. chinensis Baill, shows anti-oxidant activity and anti inflammatory effect.
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KEYWORD
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S. chinensis Baill, Reactive Oxygen Species, Oxidative DNA damage, Inflammation
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